PCR Supplies
New England Biolabs, Inc. EpiMark Hot Start Taq DNA Polymerase – 500 reactions
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA. Taq DNA polymerase possesses a 5- 3 polymerase activity (1,2,3) and a 5 flap endonuclease activity (4,5). EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
New England Biolabs, Inc. OneTaq Hot Start 2X Master Mix with Standard Buffer – 500 reactions (50 µl vol)
OneTaq Hot Start 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgCl2 and other buffer components and stabilizers listed below, requiring only the addition of primers and DNA template for robust amplification.
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient Quick-Load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands
New England Biolabs, Inc. Bst DNA Polymerase, Full Length – 500 units
Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5' to 3' polymerase activity and double-strand specific 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity.
Genscript Corporation M-MuLV Reverse Transcriptase
GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E coli strain overexpressing this construct To increase cDNA yields and get a higher percentage of longer transcripts the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity and expressed free of exogenous RNases and other nucleases The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis) making it ideal for a wide range of applications
LUNA NANOTECH INC Total Rna Purification Kit, 50 Preps
PuroSPIN™ Total RNA Purification Kit, 50 Preps
PuroSPIN™ Total RNA Purification Kitis designed to purify total RNA from bacteria, mammalian cells, yeast, or animal tissue. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The RNA is first released form the cells by the treatment with the Lysis Buffer LB-R. For RNA extraction from bacterial cell the lysis step is preceded by the treatment with the Lysozyme enzyme to disrupt bacterial cell wall. RNA is then loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water.
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatme
ABclonal Technology Bsu DNA Polymerase
Bsu DNA Polymerase, Large Fragment retains the 5?to 3? polymerase activity of DNA polymerase I but lacks the 5?to 3? exonuclease activity. This large fragment also lacks 3?to 5? exonuclease activity. Bsu DNA polymerase has strand displacement activity and is commonly used in recombinase polymerase amplification (RPA).
Roche Diagnostics LIGHTCYCLER FASTSTART DN
LightCycler FastStart DNA Master SYBR Green I - 1 kit (for 480 reactions of 20 uL final reaction volume)